Cov kauj ruam tshwj xeeb rau Extracting Chlorophyll los ntawm Cell Fragmentation Siv Ultrasonic Cell Crusher

Ultrasonic cell crusher siv cov nyhuv dispersion ntawm ultrasound hauv cov kua los tsim cavitation, yog li rhuav cov khoom los yog cov ntaub so ntswg ntawm tes hauv cov kua.
Txoj kev loj hlob ntawm kev lag luam lom neeg tau tsa qhov yuav tsum tau ua rau kev sim siv ultrasonic cell pulverizers, xws li ntsuas thiab tswj cov qauv ntsuas kub, kev txhim kho cov kev txawj ntse ntawm cov qauv ntsuas kub qis thiab tag nrho lub tshuab, thiab lwm yam.
Cov kauj ruam tshwj xeeb rau extracting chlorophyll los ntawm ultrasonic cell crushing siv lub ultrasonic cell crusher:
1. Centrifuge los yog lim cov qauv dej thiab nruab ib lub acetate fiber lim membrane ntawm lub tshuab nqus tsev lim. Ncuav ib qho ntim ntawm cov qauv dej kom pom, thiab qhov tsis zoo siab thaum pom yuav tsum tsis txhob siab dhau (txog 50kPa). Tom qab kos cov qauv dej, txuas ntxiv kos rau 1-2 feeb kom txo cov dej noo ntawm lub lim membrane. Yog tias yuav tsum khaws cia rau lub sijhawm luv luv rau 1-2 hnub, nws tuaj yeem khaws cia rau hauv lub tub yees kom khov. Yog tias yuav tsum tau khaws cia ntev (30 hnub), nws yuav tsum tau muab cia rau hauv lub tub yees txias (-20 degree ).
2. Tshem tawm cov lim membrane uas muaj phytoplankton thiab ziab nws ntawm qhov kub thiab txias hauv lub tub yees rau 6-8 teev. Txiav rau hauv me me nrog txiab thiab muab tso rau hauv 5ml los yog 10ml centrifuge raj. Ntxiv 3-4 ml ntawm 90% acetone rau hauv cov khoom tawg hauv qab cov kua dej. Muab cov fragments tso rau hauv ib lub thawv ultrasonic thiab siv ultrasonic nthwv dej los rhuav tshem cov hlwb ntawm cov qauv. (Sib piv cov kev sim ntawm cov sij hawm sib txawv thiab ntau zaus los pab txhawb kev txheeb xyuas * lub sij hawm fragmentation thiab zaus rau kev sib koom ua ke).
3. Centrifuge tus qauv raug xa mus rau cell fragmentation siv ultrasound siv lub centrifuge (3000-4000r/min) rau 10 feeb. Ncuav lub supernatant rau hauv 5ml lossis 10ml ntim ntim. Dilute rau 5ml lossis 10ml nrog 90% acetone thiab co zoo.
4. Muab lub supernatant tso rau ntawm lub spectrophotometer thiab siv 1cm optical path colorimetric phaj nyeem cov absorbance qhov tseem ceeb ntawm wavelengths ntawm 750nm, 663nm, 645nm, thiab 630nm, raws li. Siv 90% acetone ua qhov ntsuas qhov nqus nqus los kho qhov nqus ntawm cov qauv.