Txoj Cai Ua Haujlwm Ntawm Ultrasonic Cell Disruption Instrument

Ultrasound yog ib hom elastic Mechanical yoj hauv cov khoom nruab nrab. Nws yog daim ntawv yoj, yog li nws tuaj yeem siv los txheeb xyuas cov ntaub ntawv physiological thiab pathological ntawm tib neeg lub cev, uas yog kuaj ultrasound. Nyob rau tib lub sijhawm, nws kuj yog ib hom ntawm lub zog uas, thaum ib qho koob tshuaj ntawm ultrasound kis tau rau hauv lub cev, nws cuam tshuam nrog ib leeg. Ultrasound yog ib qho elastic mechanical suab nyob rau hauv cov khoom nruab nrab, thiab nws cov teebmeem ntawm cov hlwb feem ntau muaj xws li thermal teebmeem, cavitation teebmeem, thiab mechanical teebmeem.
Cov nyhuv thermal yog tias thaum ultrasound propagates nyob rau hauv nruab nrab, kev sib txhuam cuam tshuam cov molecular vibrations tshwm sim los ntawm ultrasound, ua rau ib co zog hloov mus rau hauv lub zos kub kub (42-43 degree ). Qhov no yog vim qhov ntsuas kub tseem ceeb ntawm cov ntaub so ntswg ib txwm yog 45.7 degree, thiab cov qog nqaij hlav ntau dua li cov ntaub so ntswg. Yog li ntawd, ntawm qhov ntsuas kub no, cov metabolism hauv cov qog hlwb raug cuam tshuam, thiab DNA, RNA, thiab cov protein synthesis raug cuam tshuam, yog li tua cov qog nqaij hlav tsis muaj kev cuam tshuam rau cov ntaub so ntswg. Cov nyhuv cavitation yog tsim cov vacuoles hauv cov kab mob nyob rau hauv ultrasound irradiation, uas ua rau cov neeg kho tshuab shear siab thiab turbulence nrog lub vibration thiab hnyav tawg ntawm lub vacuoles, ua rau qog los ntshav, cov ntaub so ntswg disintegration, thiab necrosis. Tsis tas li ntawd, thaum lub cavitation npuas ruptures, nws generates instantaneous kub kub (kwv yees 5000 degree) thiab high pressures (txog 500 × 104Pa) yuav ua rau thermal dissociation ntawm dej vapor los tsim. OH dawb radicals thiab. H atoms. Redox tshwm sim los ntawm. OH dawb radicals thiab. H atoms tuaj yeem ua rau polymer degradation, enzyme inactivation, Lipid peroxidation thiab cell tua. Cov nyhuv mechanical yog thawj cov nyhuv ntawm ultrasound. Thaum lub sij hawm propagation txheej txheem ntawm ultrasound, alternating compression thiab extension ntawm nruab nrab hais ua ib tug siab hloov, ua rau kev puas tsuaj rau lub cell qauv. Lub zog ntawm kev tua cov nyhuv yog ze ze rau qhov zaus thiab kev siv ntawm ultrasound.
Nyob rau tib lub sijhawm, nws kuj yog ib hom ntawm lub zog, uas tuaj yeem ua rau muaj kev hloov pauv hauv kev ua haujlwm thiab cov qauv ntawm cov kab mob los ntawm lawv cov kev sib cuam tshuam thaum qee qhov koob tshuaj ultrasound propagates nyob rau hauv lub cev, hu ua ultrasound biological nyhuv.
Lub hauv paus ntsiab lus ntawm ultrasonic Cell cuam tshuam ntsuas tsis dhau qhov tsis meej thiab nyuaj. Yooj yim muab, nws yog hloov hluav taws xob hluav taws xob mus rau lub zog suab los ntawm transducer. Lub zog no tau hloov mus rau hauv cov npuas me me los ntawm cov kua nruab nrab (xws li dej), uas tawg sai sai thiab tsim lub zog zoo li lub foob pob me, yog li ua lub luag haujlwm hauv kev rhuav tshem cov khoom xws li hlwb (feem ntau hu ua "cavitation effect"). .